ANP System

Next generation
platform for vaccine
and drug development

Unique benefits
Benefits of the Automated Nanoparticle System
Specification and components
What our customers say about the ANP System
Phosphorex uses ANP System to accelerate LNP development

Dr Julia Rashba-Step, Vice President of Research & Development and Alliance Management at Phosphorex, commented: “Having evaluated all available options for nanoparticle screening and optimization, the ANP System offers the best solution for us. We use it daily for nanoparticle formulation and process optimization as it provides robust and reproducible results - enabling us to deliver complex projects on time against aggressive deadlines. Having worked with the (now Particle Works) team for a few years, we have complete faith in their capabilities. We appreciate their willingness to work with customers to improve results, and their customer service is excellent.”

Essential documents
Can I vary parameters to change the size of lipid nanoparticles produced?
Yes, the powerful Flow Control Center software allows you to determine specific Total Flow Rates, Flow Rate Ratios and Dilution Ratio for each experiment. The graph below shows how particle size can be controlled by adjusting these parameters and also that high encapsulation efficiency is maintained throughout.

Phosphorex data
How Automated is the ANP System?
Once the system is primed and samples loaded into the Sample Loops the system can run completely autonomously until the defined experiments have been completed. The graph below shows 10 experiments being completed within 15 minutes and without human interference, including cleaning cycles.

experiment timeline
What volume samples can I make with the ANP System?
For process optimisation and low/medium-throughput screening the ANP System can make as little as 200 µl per experiment. The ANP System can also work in continuous mode to produce up to 1 liter per hour of product. The graph below shows the production of consistent size LNPs in both Protocol (200 µl to 10 ml scale) to Continuous (>10 ml to 1 liter/hour) modes. As the same microfluidic chips can be used for screening and production you can avoid the issues of variability and re-validation which are typically encountered when scaling-up.

Size vs FRR graph
What microfluidic chip geometries can I use with this system?
The standard geometry we recommend using is a cross-junction, which uses hydrodynamic flow focusing methodology – widely accepted as a highly controllably way to produce nanoparticles. However, we have a range of microfluidic geometries with a range of junction sizes and all our microfluidic chips interface with our nanoparticle generation systems, allowing you to ‘plug and play’. Our dedicated sales team will work with you to determine what microfluidic chips suit your application. If you are looking for something a little different, we have in-house design and production capabilities to produce custom microfluidic geometries.
I want to produce lipid nanoparticles and polymeric nanoparticles, is the ANP System suitable for me?
Yes! The ANP System is suitable for any self-assembly nanoparticle production process. As well as lipid nanoparticles, we have in-house experience with a range of polymeric materials, including poly(lactic-co-glycolic acid) (PLGA), polystyrene (PS), polyacrylamide, and poly(ethylene glycol) (PEG). If you are still unsure, get in contact and our Science and Applications team will discuss your needs and highlight the suitability of the ANP System.
How can I reduce the high ethanol concentration in my LNPs?
The ANP System has the ability to perform optional in-line dilutions, after nanoparticle production, with no added time to the experiment. The degree of dilution can be tailored to each experiment, allowing you to rapidly reduce the overall concentration of ethanol in your final product and continue with down-stream characterisation. Alternatively, you can perform dialysis in a buffer solution over a number of hours.
Does the ANP System have heat control?
As standard, the ANP System does not have heat control, however if this is a requirement for your nanoparticle synthesis, get in contact to discuss this need further with our dedicated Sales team to see how we could help.
What is the chemical compatibility of the ANP System?
The chemical compatibly of our wetted components has been strenuously tested and can be found in the System datasheet.
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